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T*
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T*
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T*
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(for)Tj
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T*
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T*
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T*
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T*
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T*
[(h)-5.2(B)-.5(MS)-5.1(Cs)-223.9(we)-6.4(re)-225.2(�x)-5.2(ed)-224.1(wi)-5.3(th)-224.1(95)-5.2(%)-219.4(a)-6.4(lco)-5.2(hol)-224.2(a)-6.4(n).5(d)-224.1(s).7(t)-5.3(a)-.6(in)-5.2(ed)-224.1(wi)-5.4(th)-224.1(ali)-5.3(zar)-5.2(in)]TJ
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[(gen\))-495.1(and)-494(quanti�ed)-496.2(by)-491.8(spectrometry)-496(at)-493.1(260)-492.9(and)-494(280)-164.6(nm.)]TJ
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[(g)-237.2(RNA)-236.5(template)-238.5(and)]TJ
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[(a)-532.1(M-MLV)-533.5(reverse)-535.1(transcriptase)-531.5(\(Thermo)-535.4(Scienti�c;)-533.1(Life)]TJ
T*
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[(C)-255.6(for)-256.7(2)-168.1(min)-258.1(and)]TJ
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[(C)-272.8(for)-268.2(15)-163.5(s)-271.6(and)]TJ
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()Tj
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[(PRISM)-548.5(7500)-540.1(Sequence)-549(Detection)-550.3(System)-544.4(\(Applied)-546.7(Bio-)]TJ
T*
[(systems,)-341.4(Foster)-335.8(City,)-331.7(CA\).)]TJ
1.0137 -1.1174 TD
[(Glyceraldehyde-3-phosphate)-826.9(dehydrogenase)-819.4(\()]TJ
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19.56 0 TD
(GAPDH)Tj
/F4 1 Tf
3.3867 0 TD
(\))Tj
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T*
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[(A)-5.1(C).8(AACTTT)]TJ
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[(GGTAT)-8.1(CGTGG)-9(AAGG)]TJ
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[(GCC)-5.8(A)-.2(TCAC)-5.8(GCCA)-5.9(CAGTTTC)]TJ
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(1284)Tj
49.4894 0 TD
[(LIU)-334.5(ET)-338.7(AL.)]TJ
ET
endstream
endobj
47 0 obj
<>stream
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BT
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(DD)Tj
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[(Ct)-314.4(me)18.4(th)15(od)20.8(.)]TJ
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(29)Tj
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[(Knockdown)-338(of)-330.4(TrkB)-334(and)-335.7(the)-334.8(osteogenic)-329.5(mechanism)]TJ
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[(hBMSCs)-452.7(w)15.3(e)15(r)16.2(e)-434.2(s)16.4(e)15(ed)25.4(e)15(d)-433.1(o)16.2(n)-433.1(s)16.4(i)16.1(x)16.2(-)0(w)25.8(e)15(l)16.1(l)-433.2(p)16.2(l)16.1(at)25.3(e)15(s)-432.9(a)15(t)-433.2(a)-434.2(d)16.2(e)15(n)16.2(si)26.7(t)16.1(y)-433.1(o)16.2(f)]TJ
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[(;)-678.3(G)-5.1(ene)-679.3(P)-4.1(harma,)]TJ
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T*
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[(a)-6.6(n).3(d)-402.9(c)-.9(u)-5.5(ltu)-5.5(re)-6.6(d)-397.1(f)-5.4(or)-402.8(48)-166.7(h)-402.9(a)-.9(n)-5.5(d)-397.1(t)-5.6(h).3(e)-404(t).2(ot)-5.6(al)-403(RN)-6.3(A)-398(w)-.6(a)-6.6(s)-396.9(c)-6.6(o).3(l)-5.6(l).2(ec)-6.6(te)-6.6(d)-397.1(u)-5.5(si)-5.6(ng)]TJ
T*
[(T)-6.7(R)-.8(I)-5.5(z)-1(ol)-380.1(\(I)-5.5(nv)-5.6(it)-5.7(ro)-5.6(gen)-5.6(\))-374.1(a)-6.7(s)-374(d)-5.6(e)-1(s)-5.4(c)-1(r)-5.5(i).1(b)-5.6(e)-1(d)-374.2(a)-6.7(bo)-5.6(ve)-6.7(,)-376.5(a)-1(nd)-379.9(th)-5.6(e)-375.3(r)-5.5(el)-5.7(at)-5.7(ive)-381.1(g).2(e)-6.7(n).2(e)]TJ
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(,)Tj
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[(R)-6.9(unx)-6.9(2)]TJ
/F4 1 Tf
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[(,a)387(n)388.2(d)]TJ
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[(Oste)-4.8(rix)]TJ
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[(was)-382(a)2.5(ls)3.9(o)-382.2(d)-2.1(et)3.6(ected)-382.2(b)3.7(y)]TJ
-16.0293 -1.1116 TD
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[(RT)5(-P)6.4(C)5.2(R)-.6(.)]TJ
1.0137 -1.1116 TD
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-1.0137 -1.1174 TD
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[(d)-15.3(e)10.1(tect)]TJ
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2.6149 0 TD
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(TrkB)Tj
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2.2348 0 TD
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[(mRNA)-293.6(wa)-15.2(s)]TJ
/F5 1 Tf
4.9188 0 TD
[(GGGA)-15.2(C)9.2(A)-.2(CCACGA)-15.2(A)9(CAGAAGT)]TJ
/F4 1 Tf
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(and)Tj
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[(G)-15.2(A)9.3(CGCAATC)-15.2(A)9.2(CCACCAC)-15.2(A)9.2(G)]TJ
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12.0608 0 TD
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[(,)-301.7(r).2(espe)-16(c)8.3(tive)-16(l)9.3(y.)-301.7(The)-300.5(s)-16(e)9.6(quenc)-16(e)-291.2(o).2(f)]TJ
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0 Tc
[(th)26.5(e)-284.5(s)16.4(e)15(n)16.2(se)-268.1(an)25.4(d)-283.3(a)15(n)16.2(t)16.1(i)16.1(s)0(e)25.6(n)16.2(s)16.4(e)-284.5(p)16.2(r)0(i)26.5(m)15(e)15(r)16.2(s)-283.1(f)16.2(or)]TJ
/F5 1 Tf
14.0882 0 TD
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(Runx2)Tj
/F4 1 Tf
2.7819 0 TD
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[(mRNA)-299.4(wa)-15.2(s)]TJ
/F5 1 Tf
4.9245 0 TD
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[(TG)25.9(G)15.3(T)]TJ
-21.7947 -1.1174 TD
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[(TAC)-5.8(T)1.1(G)-5.6(T)1.2(CA)-6(TG)-5.6(GC)-5.8(GGG)-5.6(TA)]TJ
/F4 1 Tf
11.5309 0 TD
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[(an)-15(d)]TJ
/F5 1 Tf
1.7567 0 TD
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[(T)-15.5(C)10.2(TCAGAT)-15.5(C)10.2(G)-.2(TTG)-15.5(A)9(A)-.5(CCT)]TJ
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[(TGCT)5.8(A.)]TJ
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[(The)-455.6(s).8(eq)-15.6(u)11(e)-.6(nce)-455.6(o)-15.6(f)-444(t).5(he)]TJ
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[(Os)25.9(t)16.1(e)15(r)16.4(i)0(x)]TJ
/F4 1 Tf
3.3003 0 TD
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[(mRNA)-455.1(pr)-15.4(i)11.1(mers)-459.8(w)-.1(as)]TJ
/F5 1 Tf
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0 Tc
[(CC)24.5(T)16.3(C)15.2(T)16.3(G)15.3(CG)24.7(G)15.3(G)15.3(A)15(C)15.2(T)16.3(CA)24.4(A)15(C)15.2(A)15(A)15(C)]TJ
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13.1552 0 TD
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[(an)-15(d)]TJ
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[(AGCCCA)-15.4(T)10.2(T).9(AGTG)-15.4(C)9.3(T)1(T)]TJ
-15.0789 -1.1174 TD
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[(G)-15.2(T)10.7(AAAGG.)-335.4(GAPDH)]TJ
/F4 1 Tf
8.6568 0 TD
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[(w)-15.8(a)8.8(s)-333.5(u).4(se)-15.8(d)-324.4(a)-.8(s)-333.5(a)-334.8(refe)-15.8(r)9.7(e)-.8(nce)-334.8(g).4(e)-15.8(n)9.6(e.)]TJ
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0 Tc
[(Total)-532.3(protein)-533.2(was)-529.3(extracted)-533.2(and)-528.6(subjected)-537.6(to)-526.5(Western)]TJ
-1.0137 -1.1116 TD
[(blotting.)-421.7(Transfected)-421(cells)-422.6(collected)-423.9(at)-418.2(0)]TJ
/F10 1 Tf
16.9163 0 TD
()Tj
/F4 1 Tf
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[(C)-416.8(i)0(n)-417(sodium)-416.7(do-)]TJ
-17.3137 -1.1116 TD
[(decyl)-382.3(sulfate)-384.4(\(SDS\))-374.6(lysis)-379.8(buffer)-382.1(\(Beyotime)-383.4(Biotechnology,)]TJ
0 -1.1174 TD
[(China\))-440.9(were)-439.5(further)-435.1(lysed)-438.6(by)-434.2(supersonic)-439.3(on)-434.2(ice)-437.7(for)-435.2(2)-168.1(min)]TJ
0 -1.1116 TD
[(discontinuously.)-499.3(Supernatants)-500.4(were)-491.4(collected)-498.8(after)-497.5(centri-)]TJ
T*
[(fuge)-316.6(for)-308.5(15)-169.2(min)-309.9(at)-308.8(4)]TJ
/F10 1 Tf
8.15 0 TD
()Tj
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[(C,)-310.8(12,000)]TJ
/F5 1 Tf
4.2852 0 TD
(g)Tj
/F4 1 Tf
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[(.)-308.7(After)-312.9(being)-312.1(denatured)-313(for)]TJ
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[(5)-168.1(min)-390.6(at)-389.4(100)-383.4(with)-391.4(loading)-389.3(buffer)-387.8(\(Huaxingbio)-8.6(,)-383.6(China\))-389(and)]TJ
0 -1.1116 TD
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T*
[(of)-330.5(20)]TJ
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(m)Tj
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[(g)-323.6(protein/lane)-334(was)-333.4(used)-327.9(for)-331.5(SDS/polyacrylamide)-332(gel)]TJ
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[(electrophoresis)-343.7(\(SDS-PAGE\).)]TJ
1.0137 -1.1116 TD
[(Each)-370.8(sample)-373(was)-373.8(run)-371.9(on)-370.8(a)-370.9(10%)-367.2(polyacrylamide)-378.8(gel)-367.5(and)]TJ
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[(transferred)-435.9(to)-434.3(a)-428.5(polyvinylidene)-433(�uoride)-435(membrane)-435.2(\()-81.7(Milli-)]TJ
T*
[(pore\).)-453.5(The)-449.2(membranes)-453.4(were)-451.1(incubated)-457.2(in)-445.8(blocking)-453.7(buffer)]TJ
0 -1.1174 TD
[(\(5%)-332.6(skimmed)-333.8(milk)-328.4(in)-330.6(Tris-buffered)-334.4(saline)-337.3(containing)-330.5(0.1%)]TJ
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[(Tween-20\))-273.4(for)-262.4(1)-168.1(h)-266(and)-263.6(incubated)-272.9(with)-264.7(the)-269.5(following)-264.6(primary)]TJ
T*
[(antibodies)-549.2(at)-544.9(4)]TJ
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()Tj
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[(C)-543.5(overnight:)-548.2(TrkB)-548(\(1:1000,)-541.1(#4603;)-548.2(Cell)]TJ
-6.8425 -1.1174 TD
[(Signaling)-477.8(Technology\),)-480(Erk1/2)-480.2(\(1:1000,)-472(#4695;)-479(Cell)-473.4(Sig-)]TJ
0 -1.1116 TD
[(naling)-428.5(Technology\),)-433.9(phospho-Erk1/2)-425.4(\(1:1000,)-431.7(#4370;)-427.2(Cell)]TJ
T*
[(Signaling)-460.6(Technology\),)-462.7(Runx2)-461.6(\(1:500,)-453.6(abs116236;)-461.4(Absin,)]TJ
0 -1.1174 TD
[(China\),)-513.4(Osterix)-505.1(\(1:300,)-511.2(abs120764;)-507.5(Absin\),)-506.2(and)-511.3(GAPDH)]TJ
0 -1.1116 TD
[(\(1:1000,)-587.2(TA-08;)-579(ZSGB-Bio,)-586.5(China\).)-582.6(Based)-590.4(on)-578.2(the)-580.6(host)]TJ
T*
[(species)-327.7(of)-318.9(primary)-327(antibody,)-318.9(HRP-conjugated)-326.2(anti-rabbit)-325.9(or)]TJ
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[(anti-mouse)-596.3(IgG)-591.6(\(ZSGB-Bio\))-591(was)-592.6(used)-592.9(for)-590.7(1)-168.1(h)-588.6(at)-591(room)]TJ
0 -1.1116 TD
[(temperature,)-349.9(The)-345.5(bands)-340.5(were)-347.4(detected)-347.8(with)-339.6(the)-344.4(chemilumi-)]TJ
T*
[(nescence)-452.2(methodology)-452.5(\(ECL)-445.5(Plus;)-448.9(Solarbio\))-448.9(by)-445.7(a)-445.8(lumino)]TJ
T*
[(imaging)-338.7(analyzer)-336.1(\(VILBER)-335.5(Fusion)-334.7(FX5,)-335.8(France\).)]TJ
/F11 1 Tf
8.9663 0 0 8.9663 59.7543 138.7842 Tm
[(p75)-335.7(and)-335.7(s100)-328.9(staining)-337.1(of)-330.4(hBMSCs)-339.3(treated)-330.4(with)-336.5(BDNF)]TJ
/F4 1 Tf
9.843 0 0 9.843 69.7323 122.3433 Tm
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/F5 1 Tf
T*
[(in)-319.1(vi)19.5(tr)20.9(o)]TJ
/F4 1 Tf
3.283 0 TD
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10.3444 0 TD
()Tj
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7.3823 0 0 6.5614 295.1999 76.8189 Tm
(2)Tj
9.843 0 0 9.843 59.7543 67.5212 Tm
-.0198 Tc
[(f)-19.8(o)-4.7(r)-401(2)-19.8(4)-166(h)-19.8(.)-393(F)-19.8(ou)-19.8(r)-396.3(e)-19.8(xp)-19.8(er)-19.8(im)-19.8(en)-19.8(ta)-19.8(l)-397.7(g)-19.8(ro)-19.8(up)-19.8(s)-396.2(w)-19.8(er)-19.8(e)-391.7(d)-19.8(es)-19.8(ig)-19.8(na)-19.8(te)-19.8(d)-391.8(a)-19.8(s)-397.3(p)-19.8(re)-19.8(-)]TJ
0 -1.1116 TD
0 Tc
[(vi)14.9(ou)20.8(sl)20.9(y)-323.6(d)0(e)19.7(s)0(c)19.9(r)0(i)20.7(b)0(e)19.7(d)0(.)]TJ
26.5234 67.9242 TD
[(Cells)-272.7(were)-278.3(�xed)-270.4(with)-276.2(4%)-274(paraformaldehyde)-278.1(on)-272.9(day)-275.2(7)-271.8(and)]TJ
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0 -1.1116 TD
[(tibody)-352.4(\(sc-271708;)-358.7(Santa)-359.1(Cruz)-357.9(Biotechnology,)-361.3(Dallas,)-358.9(TX\))]TJ
T*
[(and)-712.9(1:100)-708.3(anti-S100)-713.8(beta)-715.3(antibody)-712.9(\(ab52642;)-713.9(Abcam,)]TJ
T*
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/F10 1 Tf
8.6972 0 TD
()Tj
/F4 1 Tf
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[(C)-330.4(overnight.)-331.5(These)-331.4(were)-335.9(washed)-332.2(and)]TJ
-9.0888 -1.1174 TD
[(incubated)-249.8(in)-250(the)-252.3(dark)-253.2(for)-250.9(2)-168.1(h)-248.8(at)-251.2(37)]TJ
/F10 1 Tf
13.5065 0 TD
()Tj
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[(C,)-253.2(respectively,)-251.9(with)-253.2(488-)]TJ
-13.9039 -1.1116 TD
[(conjugated)-538.9(Af�niPure)-535.8(goat)-535.6(anti-mouse)-538.7(IgG)-534(\(H)]TJ
/F10 1 Tf
20.0899 0 TD
(+)Tj
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[(L\))-533.3(\(115-)]TJ
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[(545-003;)-371.8(Jackson)-372.5(Immuno)-377.7(Research,)-374.8(West)-376.4(Grove,)-373.8(PA\))-372.6(for)]TJ
0 -1.1174 TD
[(p75)-469.8(or)-468.7(594-conjugated)-468.4(Af�niPure)-472.5(donkey)-468.6(anti-rabbit)-475.7(IgG)]TJ
0 -1.1116 TD
(\(H)Tj
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1.0483 0 TD
(+)Tj
/F4 1 Tf
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[(L\))-585.1(\(711-585-152;)-590.3(Jackson\))-586.7(for)-585(s100,)-583.6(and)-586.2(with)-587.3(4)]TJ
/F10 1 Tf
21.357 0 TD
(�)Tj
/F4 1 Tf
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(,6-)Tj
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[(diamidino-2-phenylindole)-475.4(to)-474.6(stain)-473.3(the)-471.1(nuclei)-475.7(\(DAPI;)-474.8(Life)]TJ
0 -1.1174 TD
[(Technologies\).)-252.9(Slides)-255.3(were)-249.5(washed)-251.5(with)-253.2(PBS)-251.7(three)-249.8(times)-255.6(for)]TJ
0 -1.1116 TD
[(5)-168.1(min)-315.7(and)-321.2(then)-322.5(mounted)-321.3(with)-322.3(Fluoromount)-318.7(reagent)-322.3(\(F4680;)]TJ
T*
[(Sigma-Aldrich\))-532.6(and)-534.4(a)-526.4(coverslip)-536.4(added.)-529.6(These)-533(were)-531.7(then)]TJ
T*
[(assessed)-535.6(using)-535.3(an)-533.3(Olympus)-533.9(IX71)-535.1(microscope)-534(\(Olympus,)]TJ
0 -1.1174 TD
[(Tokyo,)-332.7(Japan\).)]TJ
/F2 1 Tf
8.9663 0 0 8.9663 310.8472 538.8093 Tm
[(In)-330.4(vivo)]TJ
/F11 1 Tf
3.2689 0 TD
(experiments)Tj
/F4 1 Tf
9.843 0 0 9.843 320.8251 522.3683 Tm
[(Six-week-old)-506(male)-503.4(BALB/c)-507.4(homozygous)-508.6(nude)-500.9(\(nu/nu\))]TJ
-1.0137 -1.1116 TD
[(mice)-324.9(were)-330.1(purchased)-324.2(from)-328.1(Peking)-324.5(University)-330(Experimental)]TJ
0 -1.1174 TD
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0 -1.1116 TD
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T*
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0 -1.1174 TD
[(me)12.7(dia)12.7(,)-400.8(acc)16.2(ord)14(ing)-389.3(to)-394(the)-384.7(exp)12.8(eri)12.7(men)11.6(tal)-384.9(des)13(ign)-389.3(\(ex)12.8(per)12.8(ime)11.5(nta)18.4(l)]TJ
0 -1.1116 TD
[(gro)14(ups)-440.8(wer)12(e)-451.5(\(I\))]TJ
/F6 1 Tf
6.975 0 TD
(b)Tj
/F4 1 Tf
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[(-T)13.9(CP,)-444.2(\(II)14.1(\))]TJ
/F6 1 Tf
4.6193 0 TD
(b)Tj
/F4 1 Tf
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[(-T)13.9(CP)]TJ
/F10 1 Tf
2.1426 0 TD
(+)Tj
/F4 1 Tf
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[(CM-)12.1(tre)12.7(ate)11.5(d)-450.4(hBM)12(SCs)13.3(,)]TJ
-15.3784 -1.1116 TD
[(\(II)14.1(I\))]TJ
/F6 1 Tf
1.8892 0 TD
(b)Tj
/F4 1 Tf
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[(-TC)11.8(P)]TJ
/F10 1 Tf
2.1484 0 TD
(+)Tj
/F4 1 Tf
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[(OM)13.3(-tr)13.9(eat)11.5(ed)-239.5(hBMSCs,)-248.8(and)-252.1(\(IV\))]TJ
/F6 1 Tf
12.6886 0 TD
(b)Tj
/F4 1 Tf
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(-TCP)Tj
/F10 1 Tf
2.4191 0 TD
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/F4 1 Tf
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[(100)-170.3(ng/)]TJ
-21.3397 -1.1116 TD
[(mL)-350.2(BDNF)]TJ
/F10 1 Tf
4.4004 0 TD
(+)Tj
/F4 1 Tf
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[(OM-treated)-356.3(hBMSCs\).)]TJ
/F6 1 Tf
9.6187 0 TD
(b)Tj
/F4 1 Tf
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[(-tri-calcium)-358.2(phosphate)]TJ
-15.125 -1.1174 TD
[(particles)-531.9(\()]TJ
/F6 1 Tf
4.2679 0 TD
(b)Tj
/F4 1 Tf
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[(-TCP,)-534(50�500)]TJ
/F6 1 Tf
6.1168 0 TD
(m)Tj
/F4 1 Tf
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[(m;)-527.7(Bicon,)-535.4(Boston,)-529.4(MA\))-534.9(were)]TJ
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[(incubated)-255.6(with)-259(each)-255.6(CM)-257.6(at)-256.9(37)]TJ
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12.0839 0 TD
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[(C)-255.6(for)-256.7(1)-162.4(h)-254.5(to)-255.8(aid)-258(cell)-254.7(adhesion.)]TJ
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[(hBMSCs)-285.7(from)-287.8(group)-281.9(II,)-287.8(III,)-283.1(and)-286.7(VI)-285.3(where)-285.1(then)-287.9(resuspended)]TJ
0 -1.1174 TD
[(and)-332.8(seeded)-338.2(onto)-332.8(the)]TJ
/F6 1 Tf
8.5186 0 TD
(b)Tj
/F4 1 Tf
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[(-TCP)-334.7(particles.)]TJ
-8.0578 -1.1116 TD
[(During)-387.7(the)]TJ
/F5 1 Tf
4.8382 0 TD
[(in)-388.2(vivo)]TJ
/F4 1 Tf
3.237 0 TD
[(experiments,)-394.7(mice)-394(were)-387.7(anesthetized)]TJ
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[(with)-374.2(sodium)-370.6(pentobarbital)-381.2(and)-373.1(four)-373(enclosed)-375.1(subcutaneous)]TJ
0 -1.1174 TD
[(transplantation)-348.7(cavities)-352.3(created)-346.5(in)-347.9(the)-344.4(dorsal)-347.5(area)-347.7(using)-345.3(he-)]TJ
0 -1.1116 TD
[(mostatic)-398.5(forceps.)]TJ
/F6 1 Tf
7.3897 0 TD
(b)Tj
/F4 1 Tf
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[(-TCP)-398.1(complexes)-400.5(from)-391.5(each)-399.6(of)-393.8(the)-396.3(four)]TJ
-7.9426 -1.1116 TD
[(groups)-737.9(were)-739.1(transplanted)-742.6(into)-736.1(individual)-738.4(subcutaneous)]TJ
0 -1.1174 TD
[(cavities)-335(\()]TJ
/F5 1 Tf
3.738 0 TD
(n)Tj
/F10 1 Tf
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(=)Tj
/F4 1 Tf
.72 0 TD
[(6)-335.2(per)-332.7(group)-333.8(at)-337.6(each)-336.2(time)-335.4(point\).)-338.6(After)-335.9(surgery,)]TJ
-5.1261 -1.1116 TD
[(animals)-391.5(were)-393.5(carefully)-388(monitored)-392.7(two)-390.2(times)-393.9(a)-388.2(day)-390.4(and)-390.4(no)]TJ
T*
[(adverse)-477.5(effects)-483.4(due)-482.5(to)-474.6(the)-482.7(surgery)-475.2(were)-485.6(observed)-477.5(in)-480.4(any)]TJ
T*
[(individual.)-327.2(Animals)-327.8(were)-330.1(sacri�ced)-331.1(at)-326(4)-329.4(and)-327(8)-323.6(weeks)-331.1(using)]TJ
0 -1.1174 TD
(CO)Tj
7.3823 0 0 6.5614 324.4535 279.6094 Tm
(2)Tj
9.843 0 0 9.843 331.9369 281.2535 Tm
[(asphyxiation)-378.6(and)-384.6(samples)-379.6(from)-385.7(each)-382.3(group)-379.9(were)-382(har-)]TJ
-2.1426 -1.1116 TD
[(vested)-331.4(and)-327(immediately)-335.4(�xed)-327.9(in)-330.6(4%)-325.8(paraformaldehyde.)-339.2(All)]TJ
T*
[(animal)-430.9(studies)-429.2(were)-433.8(performed)-428.1(in)-428.5(accordance)-438.4(with)-426(proce-)]TJ
0 -1.1174 TD
[(dures)-352(approved)-356.7(by)-353.5(the)-355.9(Animal)-355.7(Care)-359.1(and)-355.8(Ethics)-354.6(Committee)]TJ
0 -1.1116 TD
[(of)-330.5(Peking)-336(University)-335.8(Health)-338.4(Science)-334.9(Center)-338.4(\(LA2015158\).)]TJ
/F11 1 Tf
8.9663 0 0 8.9663 310.8472 215.4897 Tm
[(Histological)-333.4(study)]TJ
/F4 1 Tf
9.843 0 0 9.843 320.8251 199.0488 Tm
[(Specimens)-417.6(were)-422.3(decalci�ed)-425.9(for)-417.9(14)-422.7(days)-420.1(in)-417(10%)-419.1(EDTA)]TJ
-1.0137 -1.1174 TD
[(\(pH)-349.7(7.4\).)-350(After)-347.4(decalci�cation,)-360.3(specimens)-348.4(were)-353.2(dehydrated)]TJ
0 -1.1116 TD
[(and)-557.4(embedded)-566.5(in)-555.3(paraf�n)-562.7(wax.)-561.7(Next,)-562.9(5-)]TJ
/F6 1 Tf
17.7745 0 TD
(m)Tj
/F4 1 Tf
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[(m-thick)-563.3(tissue)]TJ
-18.3216 -1.1116 TD
[(sections)-448.7(were)-451.1(stained)-447.8(with)-449(hematoxylin)-448(and)-448(eosin)-444.3(\(H&E\))]TJ
T*
[(and)-315.5(Masson�s)-321.3(trichrome)-318.9(stains.)-316.3(Osteogenesis)-322.5(was)-316.2(evaluated)]TJ
0 -1.1174 TD
[(by)-439.9(immunohistochemical)-450.2(staining)-447.9(using)-443.2(1:200)-443.4(osteocalcin)]TJ
0 -1.1116 TD
[(\(OCN\))-330.8(antibody)-327(\(sc-30044;)-334.6(Santa)-324.6(Cruz\).)-333.6(Neurogenesis)-334.1(was)]TJ
T*
[(evaluated)-579.3(by)-578.2(immunohistochemical)-582.7(staining)-580.4(using)-575.6(1:200)]TJ
0 -1.1174 TD
[(microtubule-associated)-349.4(protein)-348.9(2)-340.9(\()-87.5(MAP2\))-341.2(\(sc-20172;)-346.1(Santa)]TJ
0 -1.1116 TD
[(Cruz\),)-235.7(1:200)-236(glial)-243.2(�brillary)-235.9(acidic)-240.7(protein)-239.5(\(GFAP\))-238.4(\(ab33922;)]TJ
T*
[(Abcam\),)-555.9(1:500)-552.8(neural/glial)-558.7(antigen)-557.5(2)-554(\(NG2\))-554.4(\(ab139406;)]TJ
0 -1.1174 TD
[(Abcam\),)-388.8(and)-390.4(1:1000)]TJ
/F6 1 Tf
8.8699 0 TD
(b)Tj
/F4 1 Tf
.5529 0 TD
[(-tubulin)-389.3(III)-389.1(\(ab78078;)-391.3(Abcam\))-391.1(anti-)]TJ
-9.4229 -1.1116 TD
[(bodies.)-506.5(Three)-504.3(�elds)-507.5(from)-506.7(three)-509(slides)-508.7(from)-506.7(each)-503.2(sampl)-7.8(e)]TJ
T*
[(were)-272.5(used)-270.3(to)-273(semiquantitatively)-279.9(assess)-273.1(the)-269.5(optical)-275.4(density)-273.9(of)]TJ
/F8 1 Tf
8.9663 0 0 8.9663 59.7543 749.7069 Tm
[(EFFECT)-338(OF)-337.5(BDNF)-330.2(ON)-334.6(BONE)-335.4(NEUROGENESIS/OSTEOGENESIS)-22070.5(1285)]TJ
ET
endstream
endobj
52 0 obj
<>stream
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BT
/F4 1 Tf
9.843 0 0 9.843 62.022 523.5022 Tm
0 0 0 rg
0 Tc
0 Tw
[(positive)-361.5(staining)-361.5(\(integrated)-361.5(optical)-361.8(density/tissu)-9.8(e)-359.4(area\))-360.3(us-)]TJ
0 -1.1116 TD
[(ing)-395.1(Image)-400.7(Pro)-394.8(Plus)-395.8(\()-81.7(Media)-400.5(Cybernetics;)-403.8(Roper)-393.6(Technolo-)]TJ
T*
[(gies,)-337.2(Lakewood)-335.8(Ranch,)-334.8(FL\).)]TJ
/F11 1 Tf
8.9663 0 0 8.9663 62.022 478.6014 Tm
[(Statistical)-336.1(analysis)]TJ
/F4 1 Tf
9.843 0 0 9.843 72 462.1605 Tm
[(Da)24.6(ta)-493.1(w)15.3(e)0(r)19.7(e)-497.6(an)19.7(a)15(l)0(y)20.7(z)0(e)24.3(d)-502.2(us)21(i)16.1(n)0(g)-491.8(S)16.3(PS)21.2(S)-502(s)16.4(of)20.9(tw)25.6(ar)19.7(e)-497.6(\()0(S)21(P)0(S)21.2(S)16.3(;)-502.3(I)0(B)19.8(M)15.3(,)]TJ
-1.0137 -1.1174 TD
[(Ar)20(m)15(o)0(n)20.8(k)16.2(,)-493(N)0(Y)24.9(\))-496.4(a)15(n)0(d)-480.3(s)0(t)20.9(a)15(ti)20.6(st)26.7(ic)19.6(al)-481.5(s)16.4(i)0(g)20.7(n)0(i)26.5(�)0(c)19.8(a)0(n)25.4(c)0(e)-482.6(w)0(a)24.6(s)-496.2(a)15(s)0(s)21.2(e)0(s)25.6(s)0(e)19.9(d)-490.7(by)]TJ
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[(-t)26.5(es)19.9(ts)-215.2(\(n)20.9(on)26.6(pa)19.7(ra)25.5(me)18.4(t)16.1(r)0(i)20.7(c)-226.9(te)19.6(st)20.9(s)16.4(\))0(.)]TJ
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[(Po)25.4(st)-445.6(ho)20.8(c)]TJ
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[(te)25.3(st)20.9(in)20.7(g)-456.1(f)16.2(or)-445.7(mu)19.6(l)16.1(t)0(i)20.6(p)0(l)26.5(e)-463(c)15(o)0(m)19.6(p)16.2(ar)19.7(is)26.7(on)20.8(s)-455.9(w)0(a)18.8(s)-455.9(c)15(a)0(r)19.7(r)0(i)26.5(e)0(d)-446.9(o)0(u)20.8(t)]TJ
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T*
[(fo)20.9(l)16.1(l)0(o)20.7(w)0(e)24.6(d)-277.6(b)16.2(y)-277.6(a)-273(N)0(e)18.8(m)15(en)19.7(y)16.2(i)-277.7(te)25.3(st)-267.1(f)16.2(o)0(r)-267.1(m)15(ul)20.7(t)16.1(i)0(p)20.7(l)0(e)-262.7(c)0(o)19.7(m)15(pa)19.7(r)16.2(i)0(s)20.9(o)0(n)26.6(s)0(.)-263.5(F)0(o)21(r)-271.8(al)19.5(l)]TJ
T*
[(te)19.5(s)16.4(t)0(s)20.9(,)-320.2(th)20.7(e)-319(t)0(h)20.7(r)16.2(es)19.9(ho)20.8(l)16.1(d)-323.6(fo)26.6(r)-323.6(s)0(t)20.9(a)15(ti)20.6(st)26.7(ic)19.6(al)-308.8(si)20.9(gn)26.6(i�)20.9(c)15(a)0(n)19.7(c)0(e)-309.8(w)0(a)18.8(s)]TJ
/F5 1 Tf
20.0841 0 TD
(p)Tj
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(<)Tj
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-.0162 Tc
[(0.)-16.2(0)8.1(5)-16.2(.)]TJ
/F8 1 Tf
8.9663 0 0 8.9663 62.022 360.3968 Tm
0 Tc
(Results)Tj
/F11 1 Tf
0 -1.8273 TD
[(Cell)-336.3(proliferation)-338.6(and)-329.4(osteogenic)-335.9(differentiation)]TJ
0 -1.2266 TD
[(of)-336.7(hBMSCs)]TJ
/F4 1 Tf
9.843 0 0 9.843 72 316.5731 Tm
[(The)-247.6(assessment)-257(of)-244.1(cell)-254.7(proliferation)-247.5(showed)-250.4(that)-253.5(hBMSCs)]TJ
-1.0137 -1.1116 TD
[(proliferated)-483.5(over)-477.8(time)-479.4(and)-482.5(that)-478.1(BDNF)-479.3(did)-475.7(not)-481.5(show)-477.4(any)]TJ
25.504 22.1345 TD
[(effect)-373(on)-370.8(proliferation.)-371.8(There)-371.9(was)-373.8(no)-365.1(signi�cant)-376.2(difference)]TJ
0 -1.1116 TD
[(betwe)-8.8(en)-504.5(the)-511.4(control)-504.4(group)-512.3(and)-505.6(the)-511.4(BDNF-treated)-509.1(group,)]TJ
T*
[(regardless)-394.3(of)-388.1(the)-384.7(concentration)-393.7(of)-388.1(added)-388(BDNF)-387.2(\(Fig.)-387.9(1A\).)]TJ
0 -1.1174 TD
[(Howev)-8.4(er,)-317.8(100)-164.6(ng/mL)-319.1(BDNF)-318(did)-320.2(signi�cantly)-321(promote)-321.2(ALP)]TJ
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[(activity)-270.8(in)-267.3(hBMSCs)-274.2(when)-265.6(compared)-272.7(to)-267.3(cells)-267.1(cultured)-270.5(in)-267.3(OM)]TJ
T*
[(only)-505.6(\()]TJ
/F5 1 Tf
2.7877 0 TD
(p)Tj
/F10 1 Tf
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(=)Tj
/F4 1 Tf
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[(0.037\).)-501.9(This)-506.6(was)-512(slightly)-504.5(higher)-509(than)-501(the)-511.4(ALP)]TJ
-4.1758 -1.1174 TD
[(activity)-443.6(observed)-437.1(in)-434.3(the)-436.6(50)-169.2(ng/mL)-434.3(BDNF)-439(group,)-435.1(although)]TJ
0 -1.1116 TD
[(there)-416.9(was)-414.1(no)-416.9(statistical)-419.2(difference.)-416.5(OM)-412.9(was)-414.1(also)-414.4(found)-414.4(to)]TJ
T*
[(promote)-321.2(osteogenic)-319.8(differentiation)-320.1(by)-319(hBMSC)-313.6(compared)-318.8(to)]TJ
0 -1.1174 TD
[(the)-350.2(control)-354.7(medium)-350.2(\(Fig.)-347.6(1B\).)-347.6(An)-348.7(analysis)-350.8(of)-347.7(gene)-352.3(expres-)]TJ
0 -1.1116 TD
[(sion)-505.4(indicated)-503.4(that)-501.2(BDNF)-508.1(signi�cantly)-505.3(increased)-504(the)-499.9(ex-)]TJ
T*
[(pression)-320.7(of)]TJ
/F5 1 Tf
4.8036 0 TD
(ALP)Tj
/F4 1 Tf
2.0965 0 TD
[(mRNA)-313.7(compared)-318.8(to)-319.1(cells)-318.9(grown)-317.4(in)-313.4(CM)-315.2(or)]TJ
-6.9001 -1.1116 TD
[(OM)-522.4(only)-522.9(without)-521.6(BDNF)-525.4(\()]TJ
/F5 1 Tf
11.6922 0 TD
(p)Tj
/F10 1 Tf
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(<)Tj
/F4 1 Tf
.72 0 TD
[(0.05\))-520.4(\(Fig.)-526.1(2A\).)-520.2(This)-518.2(was)]TJ
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[(supported)-431.4(by)-422.7(the)-425(ALP)-425.7(activity)-426.4(assay.)-425.6(Finally,)-427.2(alizarin)-427.4(red)]TJ
0 -1.1116 TD
[(staini)-8(ng)-238.3(demonstrated)-242.6(that)-242(BDNF)-243.2(promoted)-241.7(the)-235(formation)-242.9(of)]TJ
T*
[(the)-528.7(mineralized)-536.7(extracellular)-533.2(matrix)-527.6(\(ECM\))-532.7(by)-526.3(hBMSCs)]TJ
0 -1.1174 TD
[(after)-307.4(14)-295.9(days,)-302.6(when)-300.2(compared)-301.5(to)-301.8(cells)-301.6(grown)-300.1(in)-296.1(CM)-303.6(or)-295.9(OM)]TJ
0 -1.1116 TD
[(only)-338.6(\(Fig.)-330.3(2B\).)]TJ
/F11 1 Tf
8.9663 0 0 8.9663 313.0582 311.981 Tm
[(BDNF)-338(promoted)-332.9(BMSC)-335.4(osteogenesis)-333.5(via)]TJ
0 -1.2266 TD
[(the)-334.8(Erk/Runx2/Osterix)-334.8(pathway)]TJ
/F4 1 Tf
9.843 0 0 9.843 323.0361 284.5416 Tm
[(To)-412.3(clear)-14.9(ly)-411.3(deter)-13.7(mine)-415.9(the)-419.3(signal)-7.8(i)-7(n)0(g)-411.1(pathw)-13.4(ay)-412.3(resp)-11.1(onsible)]TJ
-1.0137 -1.1116 TD
[(f)-6.8(o)0(r)-486(the)-494.2(osteog)-7.7(enes)-12.3(is)-486(pro)-9(motin)-7(g)-484.9(eff)-10.2(ect)-489.6(of)-491.7(BDNF,)-494.3(BMSCs)]TJ
T*
[(w)-7.7(ere)-293.6(tr)-8.1(ansfec)-9.9(ted)-298.3(with)-299.3(siRNA-)-9.3(Tr)-9.1(kB,)-294.7(treate)-10.3(d)-294.8(with)-299.3(100)-170.3(ng/mL)]TJ
0 -1.1174 TD
[(B)-7.9(DNF,)-590(and)-586.2(then)-593.2(cultiv)-8.2(ated)-594.3(for)-585(48)-169.2(h)-588.6(t)0(o)-584.1(knockd)-13.5(own)-585.9(the)]TJ
0 -1.1116 TD
[(B)-7.9(DNF)-338.9(rec)-11.4(eptor)-340.8(g)-6.9(ene.)-343.1(In)-342(additi)-8.2(on,)-345.5(an)-337.4(siRN)-12(A)-336(was)-345(also)-345.3(used)]TJ
T*
[(a)-8(s)-444.4(negat)-8(ive)-448.1(con)-10.2(trol.)-451.6(RT-PC)-8.6(R)-445.6(resul)-12.3(ts)-445.6(showed)-452(th)-8.1(at)-447(siRNA-)]TJ
T*
[(Tr)-9.1(kB)-412.2(knocke)-8.9(d)-410(d)0(o)-8(w)0(n)-412(more)-415.7(than)-414.6(90)-8(%)-411.1(o)0(f)-411.1(the)-413.5(orig)-10.3(inal)]TJ
/F5 1 Tf
22.2901 0 TD
(TrkB)Tj
/F4 1 Tf
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[(ge)13.9(ne)19.7(.)-377.8(T)0(h)19.6(e)-376.6(ex)19.7(pr)15.1(es)19.9(si)20.9(on)-370.8(le)19.6(ve)19.7(ls)-365(of)-370.8(os)21(te)19.6(og)15.1(en)19.7(ic)-366.3(tr)20.7(an)13.9(sc)19.9(ri)20.7(pt)20.7(io)20.7(n)-381.2(f)0(a)19.7(c)0(-)]TJ
0 -1.1116 TD
[(to)15(rs)]TJ
/F5 1 Tf
1.6991 0 TD
(Runx2)Tj
/F4 1 Tf
2.7877 0 TD
(and)Tj
/F5 1 Tf
1.6818 0 TD
(Osterix)Tj
/F4 1 Tf
3.1736 0 TD
[(were)-232.2(all)-229.4(markedly)-231.3(reduced)-230(in)-232.7(siRNA-)]TJ
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[(TrkB)-242.7(knockdown)-236.6(group)-241.6(compared)-238.1(with)-241.7(those)-242.8(in)-238.5(the)-246.5(negative)]TJ
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[(control)-504.4(group)-506.6(\(Fig.)-503.1(3A�C\).)-506.2(This)-500.9(result)-508.9(indicated)-503.4(that)-501.2(by)]TJ
0 -1.1116 TD
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[(bi)5(n)5.1(d)-.6(i)5(n)5.1(g)-409.6(w)4.3(it)5(h)-409.6(T)3.9(r)5.2(k)5.1(B,)-406.1(BDNF)-403.6(pl)5(ay)5.1(ed)-403.8(a)-410.7(p)5.1(os)5.3(i)5(t)-.8(i)5(v)5.1(e)-410.7(r)5.2(ol)5(e)-405(i)-.8(n)-403.8(o)-.6(s)5.3(-)]TJ
T*
0 Tc
[(teoge)-8(nic)-373.2(differe)-10.1(ntiati)-8.3(on)-370.8(of)-370.8(BMSCs)-8.1(.)-366.3(Weste)-10(rn)-370.8(blotti)-7.1(ng)-370.8(assay)]TJ
0 -1.1174 TD
[(f)-6.8(urther)-485.9(con�rm)-8.8(ed)-481.4(and)-482.5(explor)-7.9(ed)-481.4(this)-488.3(pathwa)-9.9(y,)-482.6(and)-482.5(it)-480.5(was)]TJ
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[(obs)-8.9(erved)-468.6(that)-472.4(the)-465.4(expres)-8.7(si)-7.9(on)-463(level)-474.6(of)-462.9(phosph)-6.4(o-)-7.9(Erk1/2)-468.7(to-)]TJ
T*
[(gethe)-8(r)-433(with)-437.5(Runx2)-438.5(and)-436.4(Osterix)-441.8(evide)-8(ntly)-436.6(decre)-9(ased)-438.5(when)]TJ
0 -1.1174 TD
(the)Tj
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1.5666 0 TD
(TrkB)Tj
/F4 1 Tf
2.3384 0 TD
[(gene)-340.8(was)-339.2(knocked)-338.3(down)-339.3(\(Fig.)-341.8(3D\).)]TJ
/F11 1 Tf
8.9663 0 0 8.9663 313.0582 83.9622 Tm
[(Neurogenic)-337.8(potential)-332.9(of)-336.7(hBMSCs)]TJ
/F4 1 Tf
9.843 0 0 9.843 323.0361 67.5212 Tm
[(Analysis)-292.9(of)-284.4(neurogenesi)-9.8(s)-283.1(after)-290.1(BDNF)-295(treatment)-291.5(indicated)]TJ
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[(that)-247.7(100)-164.6(ng/mL)-244.2(BDNF)-243.2(stimulated)-245.3(hBMSCs)-239.6(to)-244.2(generate)-243.9(more)]TJ
ET
q
1 i
138.841 732.245 m
475.37 732.245 l
475.37 607.181 l
138.841 607.181 l
W n
q
336.928 0 0 125.065 138.841 607.181 cm
/Im1 Do
Q
Q
BT
/F7 1 Tf
9.845 0 0 9.845 62.022 590.3998 Tm
[(FIG.)-250.4(1.)]TJ
/F4 1 Tf
4.031 0 TD
[(Effect)-257.2(of)-249.6(BDNF)-254(on)-249.6(cell)-254.3(proliferation)-252(and)-251.8(ALP)-252.4(activity.)]TJ
/F7 1 Tf
22.2913 0 TD
(\(A\))Tj
/F4 1 Tf
1.6354 0 TD
[(Cell)-254.1(proliferation)-257.8(of)-249.6(hBMSCs)-250.3(assessed)-258.4(using)-252.6(a)-249.8(CCK-8)]TJ
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[(assay)-306.4(at)-302.8(day)-297.8(1,)-303.8(3,)-303.8(5,)-298(and)-303.6(7.)-303.8(There)-302.1(were)-306.6(no)-301.4(signi�cant)-300.3(differences)-309.3(between)-305.1(cells)-301.2(treated)-304.5(with)-304.6(50)-301.4(and)-303.6(100)-164.2(ng/mL)-306.9(BDNF,)-303.5(nor)]TJ
T*
[(be)19.9(t)16.1(w)0(e)19.1(e)15.1(n)-271.6(C)0(M)-262.9(a)0(n)25.6(d)-271.6(BD)19.2(NF)26.2(,)-274(f)0(r)21(o)16.3(m)-272.8(d)0(a)19.9(y)16.3(s)-271.5(1)-271.6(to)-261.3(7)-271.6(\()]TJ
/F5 1 Tf
17.506 0 TD
(p)Tj
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(>)Tj
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[(0.)24.4(05)21.1(\).)]TJ
/F7 1 Tf
2.5453 0 TD
[(\(B)20.1(\))]TJ
/F4 1 Tf
1.5836 0 TD
[(A)15.5(L)0(P)-262.1(a)0(c)18.7(t)16.1(iv)20.9(it)26.5(y)-271.6(a)0(t)-262.5(d)0(a)25.6(y)-271.6(7)-271.6(a)0(f)19.9(t)0(e)25.5(r)-271.6(st)21(im)25.5(ul)20.9(at)25.5(io)20.9(n)-271.6(b)0(y)-261.1(B)15.3(DN)19.4(F.)-257.6(AL)24.8(P)-271.5(a)0(c)18.7(t)0(i)26.5(v)0(i)20.9(t)0(y)-261.3(w)15.4(as)]TJ
-22.9939 -1.0135 TD
[(si)21(g)16.3(n)0(i)20.9(�)0(c)25.8(a)0(n)19.9(t)0(l)26.5(y)-392.6(hi)20.9(g)16.3(h)0(e)19.9(r)-392.6(i)16.1(n)-392.6(ce)18.7(l)16.1(l)0(s)-382.1(t)0(r)20.9(e)15.1(at)19.7(e)15.1(d)-392.6(wi)20.1(t)16.1(h)-392.6(10)21.1(0)-150.7(n)0(g)21.1(/)0(m)25.5(L)-393.7(BD)25(NF)-382.7(co)25.6(mp)19.9(a)15.1(r)0(e)19.9(d)-392.6(t)16.1(o)-392.6(th)20.9(e)-388(o)0(t)20.9(h)0(e)25.6(r)-392.6(gr)21.1(ou)26.8(ps)-381.9(\(*)]TJ
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37.6033 0 TD
(p)Tj
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(<)Tj
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[(0.)24.4(05)26.8(\).)-384.4(A)15.5(L)0(P)20.1(,)-389.2(al)19.7(k)16.3(a)0(l)19.7(i)0(n)26.6(e)-393.7(ph)26.8(os)21.2(-)]TJ
-38.9681 -1.0077 TD
[(ph)21.1(a)15.1(t)0(a)19.7(s)0(e)25.8(;)-323.6(BD)25(NF)20.4(,)-320.1(b)0(r)21.1(a)15.1(in)20.9(-d)26.8(er)19.9(iv)26.6(ed)-314.1(n)16.3(e)0(u)19.9(r)0(o)26.8(t)0(r)20.9(o)0(p)26.8(h)0(i)20.9(c)-324.6(f)16.3(a)0(c)18.7(t)16.1(or)21.1(;)-323.7(C)15.3(M,)-310.4(cu)19.9(l)16.1(t)0(u)20.9(r)0(e)-308.4(m)0(e)18.7(d)16.3(iu)20.9(m)15.1(;)-323.7(hB)25.8(MS)20.4(Cs)26(,)-325.9(h)16.3(um)19.9(a)15.1(n)-323.5(bo)21.1(n)16.3(e)-324.6(m)15.1(e)0(s)20.1(e)0(n)25.6(c)0(h)19.9(y)0(m)25.6(a)0(l)-314.3(s)16.4(te)19.7(m)-318.9(c)0(e)18.7(l)16.1(ls)21(.)]TJ
ET
q
1 i
62.589 281.707 m
300.529 281.707 l
300.529 162.992 l
62.589 162.992 l
W n
q
238.023 0 0 118.715 62.589 162.992 cm
/Im2 Do
Q
Q
BT
/F7 1 Tf
9.845 0 0 9.845 62.022 146.211 Tm
[(FIG.)-607.4(2.)]TJ
/F5 1 Tf
4.3823 0 TD
(ALP)Tj
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2.3898 0 TD
[(gene)-605.2(expression)-605.2(and)-603(mineralized)-610.4(nodule)]TJ
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[(formation)-334.1(after)-335.7(stimulation)-330.6(by)-336(BDNF.)]TJ
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15.8878 0 TD
(\(A\))Tj
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1.716 0 TD
[(Three)-336.7(days)-327.4(after)]TJ
-17.6039 -1.0135 TD
[(100)-170(ng/mL)-272.3(BDNF)-277.1(treatment,)-276.6(RT-PCR)-274.8(revealed)-283.4(signi�cantly)]TJ
T*
(more)Tj
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2.3783 0 TD
(ALP)Tj
/F4 1 Tf
2.0961 0 TD
[(mRNA)-318.7(expression)-317.3(compared)-323.6(to)-313.1(the)-315.3(other)-323(groups)]TJ
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(\(*)Tj
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(p)Tj
/F10 1 Tf
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(<)Tj
/F4 1 Tf
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